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Image Search Results
Journal: International journal of molecular medicine
Article Title: SphK1 modulates cell migration and EMT-related marker expression by regulating the expression of p-FAK in colorectal cancer cells.
doi: 10.3892/ijmm.2017.2921
Figure Lengend Snippet: Figure 1. Sphingosine kinase 1 (SphK1) affects colorectal cancer cell migration. The suppression of SphK1 by SKI-Ⅱ (20 µM) for 48 h (SK group) significantly decreased the number of cells penetrating through the porous membrane. (A) Caco2 cell line. (B) HT29 cell line. (C) RKO cell line. (D) HCT116 cell line. The images are representative of cell penetration (crystal violet staining, magnification, x200) and the data are shown as the means ± SD (*P<0.05 vs. N group).
Article Snippet: The cells in the upper chamber were wiped off and the cells migrating to the lower side of the upper chamber were stained with 0.1% crystal violet (Beyotime, Shanghai, China) for 30 min. Random fields were scanned (6 fields/filter) under a fluorescent
Techniques: Migration, Membrane, Staining